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Microbiology Independent Research Journal (MIR Journal)

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Enhanced CD8+ T-cell response in mice immunized with NS1-truncated influenza virus

https://doi.org/10.18527/2500-2236-2020-7-1-24-33

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Аннотация

Influenza viruses with truncated NS1 protein stimulate a more intensive innate immune response compared to their wild type counterparts. Here, we investigate how the shortening of the NS1 protein influence the immunogenicity of the conserved T-cellular epitopes of influenza virus. Using flow cytometry, we showed that the intraperitoneal immunization of mice with influenza virus encoding 124 N-terminal amino acid residues of the NS1 protein (A/PR8/NS124) induced higher levels of CD8+ T-cells recognizing immunodominant (NP366-374) and sub-immunodominant (NP161-175, NP196-210, HA323-337, HA474-483, NA427-433) epitopes compared to immunization with the virus expressing full-length NS1 (A/PR8/full NS). It is noteworthy that the response to the immunodominant influenza epitope NP366-374 was achieved with the lower immunization dose of A/PR8/NS124 virus compared to the reference wild type strain. Despite the fact that polyfunctional CD8+ effector memory T-lymphocytes simultaneously producing two (IFNγ and TNFα) or three (IFNγ, IL2, and TNFα) cytokines prevailed in the immune response to both viruses, the relative number of such T-cells was higher in A/PR8/NS124-immunized mice. Furthermore, we have found that polyfunctional populations of lymphocytes generated upon the immunization of mice with the mutant virus demonstrated an increased capacity to produce IFNγ compared to the corresponding populations derived from the A/PR8/full NS-immunized mice. Therefore, immunization with the attenuated influenza virus encoding truncated NS1 protein ensures a more potent CD8+ T-cell immune response.

Об авторах

K. A. Vasilyev
Smorodintsev Research Institute of Influenza
Россия

Kirill Vasilyev 


St. Petersburg



A.-P. S. Shurygina
Smorodintsev Research Institute of Influenza
Россия

St. Petersburg



M. A. Stukova
Smorodintsev Research Institute of Influenza
Россия

St. Petersburg



A. Y. Egorov
Smorodintsev Research Institute of Influenza; University of Natural Resources and Life Sciences
Россия

St. Petersburg


Vienna, Austria



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Дополнительные файлы

1. Table 2S. Cellular polyfunctionality index and the integrated IFNγ MFI of influenza-specific CD8+ EM T-lymphocytes on the 8th day after the i.p. immunization with А/PR8/full NS and А/PR8/NS124 strains
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Для цитирования:


Vasilyev K.A., Shurygina A.S., Stukova M.A., Egorov A.Y. Enhanced CD8+ T-cell response in mice immunized with NS1-truncated influenza virus. Microbiology Independent Research Journal (MIR Journal). 2020;7(1):24-33. https://doi.org/10.18527/2500-2236-2020-7-1-24-33

For citation:


Vasilyev K.A., Shurygina A.S., Stukova M.A., Egorov A.Y. Enhanced CD8+ T-cell response in mice immunized with NS1-truncated influenza virus. Microbiology Independent Research Journal (MIR Journal). 2020;7(1):24-33. https://doi.org/10.18527/2500-2236-2020-7-1-24-33

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