First report of the fungus Diaporthe phaseolorum on sunflower in Russia

Полный текст:


Fungi of the ascomycete genus Diaporthe have been identified worldwide. Typically, Diaporthe species are saprobes, endophytes, or plant pathogens. The distinction between the species of this genus has historically been based on the combination of the morphological information, cultural characteristics, and host affiliation. The correct identification of the Diaporthe species should be carried out based on a combination of molecular genetic traits. A comprehensive analysis of Diaporthe species in the Russian Federation using molecular phylogeny methods has never been accomplished.
The goal of this study was the identification of the isolate Diaporthe sp. MF 16-010, extracted from stems of Helianthus annuus L. that was collected in the Krasnodar region of the Russian Federation. According to the morphology data and DNA sequence analyses of the nuclear ribosomal internal transcribed spacer (ITS) region as well as of the translation elongation factor-1α (EF-1α), and ß-tubulin genes, the isolate MF 16-010 was identified as Diaporthe phaseolorum (Cooke & Ellis) Sacc. To the best of our knowledge, this isolate represents the first report of Diaporthe phaseolorum associated with sunflower in the Russian Federation. The development of the stem lesions as a result of the artificial inoculation of MF 16-010 to sunflower proved that this isolate is pathogenic for sunflower.

Ключевые слова

Об авторах

M. M. Gomzhina
All-Russian Institute of Plant Protection

Maria Gomzhina 

St. Petersburg

Ph. B. Gannibal
All-Russian Institute of Plant Protection
St. Petersburg

Список литературы

1. Udayanga D, Liu X, McKenzie EHC, Chukeatirote E, Bahkali AHA, Hyde KD. The genus Phomopsis: Biology, applications, species concepts and names of common phytopathogens. Fungal diversity 2011; 50(1), 189-225. doi: 10.1007/s13225-011-0126-9.

2. Santos JM, Correia VG, Phillips AJL. Primers for mating-type diagnosis in Diaporthe and Phomopsis: their use in teleomorph induction in vitro and biological species definition. Fungal Biology 2010; 114(2-3), 255-70. doi: 10.1016/j.funbio.2010.01.007.

3. Santos L, Alves A, Alves R. Evaluating multi-locus phylogenies for species boundaries determination in the genus Diaporthe. PeerJ 2017, 1-26. doi: 10.7717/peerj.3120.

4. Kolomiets T, Mukhina Z, Matveeva T, Bogomaz D, Berner DK, Cavin CA, Castlebury LA. First Report of Stem Canker of Salsola tragus Caused by Diaporthe eres in Russia. Plant disease 2009; 93 (1), 110. doi: 10.1094/PDIS-93-1-0110B.

5. Samson RA, Hoekstra ES, Frisvad JC, Filtenborg O. Introduction to food- and airborne fungi. 6th edition. Utrecht: Centraal bureau voor schimmel cultures; 2000. ISBN-10: 9070351420.

6. Doyle JJ, Doyle JL. Isolation of plant DNA from fresh tissue. Focus 1990; 12, 13-15.

7. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 1993; 2, 113–18. doi: 10. 1111/j.1365-294X.1993.tb00005.x.

8. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA et al. (eds.), PCR Protocols: A guide to Methods and Applications. Academic Press, San Diego, USA. 1990, 315-322.

9. Aveskamp MM, Verkley GJM, de Gruyter J, Murace MA, Perelló A, Woudenberg JHC, Groenewald JZ, Crous PW. DNA phylogeny reveals polyphyly of Phoma section Peyronellaea and multiple taxonomic novelties. Mycologia 2009; 101(3), 363-82. doi: 10.3852/08-199.

10. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999; 9, 553–56. doi: 10.2307/3761358.

11. Boyle JS, Lew AM. An inexpensive alternative to glassmilk for DNA purification. Trends Genet 1995, 11(1), 8. doi: 10.1016/S0168-9525(00)88977-5.

12. Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the USA, Biochemistry 1977; 74(12), 5463-67. PMID: 271968.

13. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res 1997; 24, 4876-82. PMID: 9396791.

14. Stamatakis A. RAxML-VI-HPC: Maximum likelihoodbased phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 2006; 22(21), 2688-90. doi: 10.1093/bioinformatics/btl446.

15. National center for biotechnology information. Available:

16. Boerema GH, de Gruyter J, Noordeloos ME, Hamers MEC. Phoma identification Manual. Wallingford: CABI Publishing; 2004. ISBN-10: 0851997430.

17. Miric E, Aitken EAB, Goulter KC. Identification in Australia of the quarantine pathogen of sunflower Phoma macdonaldii (Teleomorph: Leptosphaeria lindquistii). Australian Journal of Agricultural Research 1999; 50, 325-32. doi: 10.1071/A98072.

18. Schneiter AA, Miric JF, Berglund DR. Description of sunflower growth stages. Crop. Sci. 1981; 21(6), 901-3. doi: 10.2135/cropsci1981.0011183X002100060024x.

19. Thompson SM, Tan YP, Young AJ, Neate SM, Aitken EAB, Shivas RG. Stem cankers on sunflower (Helianthus annuus) in Australia reveal a complex of pathogenic Diaporthe (Phomopsis) species. Persoonia 2011; 27, 80-89. doi: 10.3767/003158511X617110.

20. Перечень карантинных объектов (вредителей растений, возбудителей болезней растений и растений (сорняков)). Доступен:

21. Gomes RR, Glienke C, Videira SIR, Lombard L, Groenewald JZ, Crous PW. Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. Persoonia 2013; 31, 1-41. doi: 10.3767/003158513X666844.

22. Dissanayake AJ, Camporesi E, Hyde KD, Zhang W, Yan JY, Li XH. Molecular phylogenetic analysis reveals seven new Diaporthe species from Italy. Mycosphere 2017; 8(5), 853-77. doi: 10.5943/mycosphere/8/5/4.

23. Mathew F, Alananbeh K, Balbyshev N, Heitkamp E, Castlebury L, Gulya T, Markell S. Reevaluation of Phomopsis species affecting sunflowers in the United States. Phytopathology 2011, 101(S115).

24. Mathew FM, Alananbeh KM, Jordah JG, Meyer SM, Castlebury LA, Gulya TJ, Markell SG. Phomopsis Stem Canker: A Reemerging Threat to Sunflower (Helianthus annuus) in the United States. Phytopathology 2015; 105(7), 990-7. doi: 10.1094/PHYTO-11-14-0336-FI.

25. Mathew FM, Rashid KY, Gulya TJ, Markell SG. First Report of Phomopsis Stem Canker of Sunflower (Helianthus annuus) Caused by Diaporthe gulyae in Canada. Disease notes 2015; 99(1), 160. doi: 10. 1094/PDIS-08-14-0858-PDN.

26. Thompson SM, Tan YP, Shivas RG, Neate SM, Morin L, Bissett A, Aitken EAB. Green and brown bridges between weeds and crops reveal novel Diaporthe species in Australia. Persoonia 2015; 35, 39-49. doi: 10.3767/003158515X687506.

27. Udayanga D, Liu X, Crous PW, McKenzie EHC, Chukeatirote E, Hyde KD. A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis). Fungal diversity 2012; 56(1), 157-71. doi: 10.1007/s13225-012-0190-9.

28. Santos JM, Vrandečić K, Ćosić J, Duvnjak T, Phillips AJL. Resolving the Diaporthe species occurring on soybean in Croatia. Persoonia 2011; 27, 9–19. doi: 10.3767/003158511X603719.

29. Olson TR, Kontz B, Markell SG, Gulya TJ, Mathew FM. First Report of Diaporthe stewartii Causing Phomopsis Stem Canker of Sunflower (Helianthus annuus) in Minnesota. Disease notes 2017; 101(2), 382. doi: 10.1094/PDIS-08-16-1122-PDN.

30. Mattei AS, Severo CB, Guazzelli LC, Oliveira FM, Gené J, Guarro J, Cano J, Severo LC. Cutaneous infection by Diaporthe phaseolorum in Brazil. Medical Mycology Case Reports 2013; 2, 85-7. doi: 10.1016/j.mmcr.2013.03.001.

Для цитирования:

Gomzhina M.M., Gannibal P.B. First report of the fungus Diaporthe phaseolorum on sunflower in Russia. Microbiology Independent Research Journal (MIR Journal). 2018;5(1):65-70.

For citation:

Gomzhina M.M., Gannibal P.B. First report of the fungus Diaporthe phaseolorum on sunflower in Russia. Microbiology Independent Research Journal (MIR Journal). 2018;5(1):65-70.

Просмотров: 41

Creative Commons License
Контент доступен под лицензией Creative Commons Attribution 4.0 License.

ISSN 2500-2236 (Online)