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Microbiology Independent Research Journal (MIR Journal)

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The use of microarrays for the identification of the origin of genes of avian influenza viruses in wild birds

https://doi.org/10.18527/2500-2236-2017-4-1-21-30

Полный текст:

Аннотация

Forty-two strains of avian influenza viruses were isolated from the wild waterfowls’ feces in the city of Moscow. These viruses, as well as reference strains and some experimental reassortants, were analyzed by microarrays. The microarrays contained 176 probes to the different segments of influenza virus genome. The microarray helps to determine 1) the hemagglutinin and neuraminidase proteins subtype; 2) the primary structure of the C-terminal sequence of the viral NS1 protein, which serves as a ligand for the PDZ domain; 3) the presence of stop codons in the reading frame of PB1-F2 as well as the N66S substitution in the PB1-F2 viral protein; 4) the presence of the polybasic site for hemagglutinin cleavage. The viruses of the H3N1, H3N6, H3N8, H4N6, H1N1, H5N3, and H11N9 subtypes were identified from the group of wild birds’ isolates. All isolates contained the ESEV sequence at the C-terminus of the NS1 protein and the full-length reading frame for the PB1-F2 protein. The replacement of N66S in PB1-F2 was found in six strains. However, the presence of the ESEV sequence (ligand of PDZ domain) in the NS1 virus protein and the N66S substitution in PB1-F2 did not lead to the pathogenicity of these viruses for mice. All isolates demonstrated high yield growth in chicken embryos and were infectious and immunogenic for mice, but did not induce any clinical symptoms.

Ключевые слова


Об авторах

R. N. Heydarov
Engelhardt Institute of Molecular Biology, RAS
Россия
Moscow


N. F. Lomakina
Chumakov Federal scientific center for the research and development of immune-and-biological products
Россия
Moscow


E. Yu. Boravleva
Chumakov Federal scientific center for the research and development of immune-and-biological products
Россия
Moscow


I. S. Kholodilov
Chumakov Federal scientific center for the research and development of immune-and-biological products
Россия
Moscow


A. S. Gambaryan
Chumakov Federal scientific center for the research and development of immune-and-biological products
Россия

Alexandra Gambaryan

Moscow



V. M. Mikhailovich
Engelhardt Institute of Molecular Biology, RAS
Россия
Moscow


E. E. Fesenko
Institute of Cell Biophysics, RAS
Россия
Pushchino


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Для цитирования:


Heydarov R.N., Lomakina N.F., Boravleva E.Y., Kholodilov I.S., Gambaryan A.S., Mikhailovich V.M., Fesenko E.E. The use of microarrays for the identification of the origin of genes of avian influenza viruses in wild birds. Microbiology Independent Research Journal (MIR Journal). 2017;4(1):21-30. https://doi.org/10.18527/2500-2236-2017-4-1-21-30

For citation:


Heydarov R.N., Lomakina N.F., Boravleva E.Yu., Kholodilov I.S., Gambaryan A.S., Mikhailovich V.M., Fesenko E.E. The use of microarrays for the identification of the origin of genes of avian influenza viruses in wild birds. Microbiology Independent Research Journal (MIR Journal). 2017;4(1):21-30. https://doi.org/10.18527/2500-2236-2017-4-1-21-30

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