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Microbiology Independent Research Journal (MIR Journal)

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Development of the A/H6N1 influenza vaccine candidate based on A/Leningrad/134/17/57 (H2N2) master donor virus and the genome composition analysis using high resolution melting (HRM)

https://doi.org/10.18527/2500-2236-2016-3-1-61-67

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Аннотация

The avian influenza viruses of H6N1subtype present a potential danger for humans. The cold-adapted (ca) reassortant influenza virus А/17/herring gull/Sarma/2006/887 (H6N1) was obtained in chicken embryos by the genetic reassortment based on the coldadapted A/Leningrad/134/17/57 (H2N2) master strain. The genome composition of the obtained reassortant was analyzed by means of real-time PCR with the high resolution melting (HRM) analysis using the intercalating fluorescent dye EvaGreen. Analysis of the gene segments showed that the reassortant А/17/herring gull/Sarma/2006/887 (H6N1) contains the internal proteins coding genes (PB2, PB1, PA, NP, M, and NS) of the master donor virus and the surface antigens coding genes of the A/herring gull/Sarma/51c/2006 (H6N1) avian influenza virus. The study of the phenotypic properties showed that the virus А/17/herring gull/Sarma/2006/887 (H6N1) is temperature sensitive (ts), ca in chicken embryos, and attenuated in mice when administered intranasally. This reassortant can be recommended as a live influenza vaccine candidate for humans.

Ключевые слова


Об авторах

Yu. A. Desheva
Institute of Experimental Medicine; Saint Petersburg State University
Россия

Yulia A. Desheva

Saint Petersburg



T. A. Smolonogina
Institute of Experimental Medicine
Россия
Saint Petersburg


G. O. Landgraf
Saint Petersburg State University
Россия
Saint Petersburg


L. G. Rudenko
Institute of Experimental Medicine
Россия
Saint Petersburg


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Для цитирования:


Desheva Y.A., Smolonogina T.A., Landgraf G.O., Rudenko L.G. Development of the A/H6N1 influenza vaccine candidate based on A/Leningrad/134/17/57 (H2N2) master donor virus and the genome composition analysis using high resolution melting (HRM). Microbiology Independent Research Journal (MIR Journal). 2016;3(1):61-67. https://doi.org/10.18527/2500-2236-2016-3-1-61-67

For citation:


Desheva Yu.A., Smolonogina T.A., Landgraf G.O., Rudenko L.G. Development of the A/H6N1 influenza vaccine candidate based on A/Leningrad/134/17/57 (H2N2) master donor virus and the genome composition analysis using high resolution melting (HRM). Microbiology Independent Research Journal (MIR Journal). 2016;3(1):61-67. https://doi.org/10.18527/2500-2236-2016-3-1-61-67

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