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Development of the A/H6N1 influenza vaccine candidate based on A/Leningrad/134/17/57 (H2N2) master donor virus and the genome composition analysis using high resolution melting (HRM)

https://doi.org/10.18527/2500-2236-2016-3-1-42-48

Abstract

The cold-adapted reassortant influenza virus А/17/herring gull/Sarma/2006/887 (H6N1) was developed in chicken embryos by genetic reassortment based on the A/Leningrad/134/17/57 (H2N2) master donor virus. The genome composition of the obtained reassortant was analyzed by means of real time PCR with the high resolution melting (HRM-analysis) using an intercalating fluorescent dye EvaGreen. Analysis of the DNA copies melting curves of RNA gene fragments (PB2, PB1, PA, NP, M, NS) showed that the reassortant influenza virus А/17/herring gull/2006/887(H6N1) contained the internal proteins coding genes from the master donor virus and the surface antigens coding genes from the A/herring gull/Sarma/51c/2006 (H6N1) avian influenza virus. The study of phenotypic properties showed that the А/17/herring gull/Sarma/2006/887 (H6N1) is temperature sensitive (ts) and coldadapted (ca) in chicken embryos and attenuated in mice when administered intranasally. This reassortant can be recommended as a live influenza vaccine candidate for humans.

About the Authors

Yu. A. Desheva
Institute of Experimental Medicine; Saint Petersburg State University
Russian Federation

Yulia A. Desheva

Saint Petersburg



T. A. Smolonogina
Institute of Experimental Medicine
Russian Federation
Saint Petersburg


G. O. Landgraf
Saint Petersburg State University
Russian Federation
Saint Petersburg


L. G. Rudenko
Institute of Experimental Medicine
Russian Federation
Saint Petersburg


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Review

For citations:


Desheva Yu.A., Smolonogina T.A., Landgraf G.O., Rudenko L.G. Development of the A/H6N1 influenza vaccine candidate based on A/Leningrad/134/17/57 (H2N2) master donor virus and the genome composition analysis using high resolution melting (HRM). Microbiology Independent Research Journal (MIR Journal). 2016;3(1):42-48. (In Russ.) https://doi.org/10.18527/2500-2236-2016-3-1-42-48

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ISSN 2500-2236 (Online)