ISSN 2500-2236
DOI-prefix: 10.18527/2500-2236

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Volume 8, Number 1, 2021
... Viruses Four virus variants with different combinations of secondary structures in the two NS RNA regions (82-148 and 497-564) were previously obtained by reverse genetics, ...
Authors: Irina L. Baranovskaya, Mariia V. Sergeeva, Aleksandr S. Taraskin, Alexey A. Lozhkov, Andrey V. Vasin
Volume 3, Number 1, 2016
... C-DNAs were synthesized using M-MuLV reverse transcriptase (MBI). The M gene and three overlapping parts of the HA gene were amplified by PCR using specific primers (sequence available upon request). ...
Authors: Andrea Wolkerstorfer, Dietmar Katinger, Julia Romanova
Volume 6, Number 1, 2019
... The cDNA corresponding to both NP genes was amplified using forward primers with a NdeI restriction site and the reverse primers containing the XhoI restriction site (Table 1). ...
Authors: Nikita D. Yolshin, Aram A. Shaldzhyan, Sergey A. Klotchenko
Том 6, номер 1, 2019
... Scientists are concentrating their efforts on studying the mechanism of this process in order to find a way to stop or reverse it. ...
Authors: Александр Живич
Volume 6, Number 1, 2019
... Scientists are concentrating their efforts on studying the mechanism of this process in order to find a way to stop or reverse it. ...
Authors: Alexander Zhivich
Volume 5, Number 1, 2018
... This inactivated vaccine was based on reassortants engineered by reverse genetics, which contain the H5 hemagglutinin (HA) and N1 neuraminidase (NA) from H5N1 viruses and the remaining genes from A/Puerto ...
Authors: Elizaveta Boravleva, Alexandra Gambaryan
Volume 7, Number 1, 2020
... The strains were obtained by the method of reverse genetics [17] assembled in developing chicken embryos and purified by fractionation in a sucrose density gradient ...
Authors: Kirill A. Vasilyev, Anna-Polina S. Shurygina, Marina A. Stukova, Andrej Y. Egorov
Volume 2, Number 1, 2015
... Confirmation of influenza infection Real-time multiplex reverse transcription (RT)-PCR was performed according to the procedures ...
Authors: Anna A. Sominina, Maria M. Pisareva, Mikhail P. Grudinin, Mikhail Yu. Eropkin, Nadezhda I. Konovalova, Vera F. Sukhovetskaya, Olga I. Afanasieva, Lubov V. Voloshuk, Andrey B. Komissarov, Zhanna V. Buzitskaya, Artem V. Fadeev, Kirill A. Stolyarov, Elizaveta A. Smorodintseva, Veronika S. Afanasieva, Marina A. Yanina, Vitalii V. Gonchar, Ludmila V. Osidak, Alexey A. Go
Volume 4, Number 1, 2017
... The Vietnam/1203/04-PR8/CDC-RG (H5N1) (VN-PR) virus was generated by reverse genetics in the Influenza branch of the Centers for Disease Control and Prevention (CDC, USA). ...
Authors: Rustam N. Heydarov, Natalia F. Lomakina, Elizaveta Yu. Boravleva, Ivan S. Kholodilov, Alexandra S. Gambaryan, Vladimir M. Mikhailovich, Eugene E. Fesenko
Volume 5, Number 1, 2018
... genetics. ...
Authors: Andrej Egorov
Volume 3, Number 1, 2016
... genetics [22, 23]), only inactivated, live ca, and vector vaccines have broad practical application against the equine ...
Authors: Kaissar Tabynov
Volume 5, Number 1, 2018
... The revision of the genus, carried out over the past few years within the framework of a polyphasic approach to the systematics of microorganisms and based on a comprehensive analysis of molecular genetics ...
Authors: Maria M. Gomzhina, Philipp B. Gannibal
Volume 1, number 1, 2014
... All H5N1 recombinant viruses used in the present study were obtained by reverse genetics on Vero cells. ...
Authors: Mariia Sergeeva, Artem Krokhin, Mikhail Matrosovich, Tatyana Matrosovich, Markus Wolschek, Oleg Kiselev, Julia Romanova
Volume 4, Number 1, 2017
... The construction of vaccine reassortants by reverse genetics methods allows for the cloning of the HA sequence identical to that of the virus present in the human swab, and for the rescue of the virus ...
Authors: Julia Romanova
Volume 1, number 1, 2014
... Research papers from the journal “Molecular genetics, microbiology and virology” (Molekulyarnaya genetika, mikrobiologiya i virusologiya) are translated in English, but this journal is not ...
Authors: Dr. J. Romanova
Volume 3, Number 1, 2016
... Reverse transcription (RT) was performed using the reverse transcriptase of the leukemia Molony mouse virus (M-MulV, SibEnzyme) and random hexamers. ...
Authors: Yulia A. Desheva, Tatiana A. Smolonogina, Galina O. Landgraf, Larisa G. Rudenko
Volume 2, Number 1, 2015
... Large scale studies on population genetics and sensitivity to influenza prove that the anti-epidemic actions in different regions of the country should be planned in accordance to the specific genetic ...
Authors: O. I. Kiselev, A. B. Komissarov, O.S.Konshina, M.N.Dmitrieva, E.G.Deyeva, T.V.Sologub, V.I.Pokrovskiy
Volume 2, Number 1, 2015
... Reverse transcription of the RNA was performed by Reverta-L kit (InterLabService, Russia). The amplification of cDNA was performed by the standard method using the original primers. ...
Authors: Mikhail P. Grudinin, Maria M. Pisareva, Andrey B. Komissarov, Anna A. Kosheleva, Zhanna V. Buzitskaya, Marina A. Stukova, Alexandra V. Prokopetz, Daria M. Danilenko, Nadezhda I. Konovalova, Tamara G. Lobova, Anna A. Sominina, Oleg I. Kiselev
Volume 3, Number 1, 2016
... The reverse transcription of RNA was performed for 40 min at 37°C using the random hexamer primers and commercial kit Reverta-L (Interlabservice, Russia). ...
Authors: Evgeniy V. Sorokin, Tatyana R. Tsareva, Anna A. Sominina, Maria M. Pisareva, Andrey B. Komissarov, Аnna А. Kosheleva
Том 3, номер 1, 2016
... ОТ проводили с использованием ревертазы мышиного вируса лейкемии Молони (M-MulV Reverse Transcriptase, Сиб­Энзим) и случайных гексамеров. ...
Authors: Ю. А. Дешева, T. А. Смолоногина, Г. О. Ландграф, Л. Г. Руденко