ISSN 2500-2236
DOI-prefix: 10.18527/2500-2236

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Volume 5, Number 1, 2018
... 10.0. The relationship between the quantitative traits was evaluated using the linear Pearson correlation coefficient (r) at a significance level of p<0.01 or p<0.05. The coefficient of variation (CV, %) was defined as the ratio of the standard deviation to the mean. ...
Authors: Aleksandra S. Orina, Olga P. Gavrilova, Tatiana Yu. Gagkaeva
Том 5, номер 1, 2018
... quantitative PCR has been adapted for the analysis of the target DNA of Alternaria spp., Bipolaris sorokiniana (B. sorokiniana), Fusarium graminearum (F. graminearum), F ...
Authors: А. С. Орина, О. П. Гаврилова, Т. Ю. Гагкаева
Volume 3, Number 1, 2016
... Reed – Muench method [14]. PCR with HRM-analysis The virus RNA was isolated from 80 μl of the virus containing allantoic ...
Authors: Yulia A. Desheva, Tatiana A. Smolonogina, Galina O. Landgraf, Larisa G. Rudenko
Том 3, номер 1, 2016
... ). Секвенирование по Сэнгеру Для частичного секвенирования были получены ДНК-копии сегментов РНК PB2, PB1, PA, NP и NA с помощью набора OneStep RT-PCR Kit (Qiagen, Нидерланды ...
Authors: Ю. А. Дешева, T. А. Смолоногина, Г. О. Ландграф, Л. Г. Руденко
Volume 2, Number 1, 2015
... Real-time multiplex reverse transcription (RT)-PCR was performed according to the procedures described in the manufacturer’s instructions (InterLabService, Russia). RNA ...
Authors: Anna A. Sominina, Maria M. Pisareva, Mikhail P. Grudinin, Mikhail Yu. Eropkin, Nadezhda I. Konovalova, Vera F. Sukhovetskaya, Olga I. Afanasieva, Lubov V. Voloshuk, Andrey B. Komissarov, Zhanna V. Buzitskaya, Artem V. Fadeev, Kirill A. Stolyarov, Elizaveta A. Smorodintseva, Veronika S. Afanasieva, Marina A. Yanina, Vitalii V. Gonchar, Ludmila V. Osidak, Alexey A. Go
Volume 5, Number 1, 2018
... ]; βtub2Fw/βtub4Rd [9]; EF1-728F/EF1-986R [10]. Each PCR mixture (25 μl) contained: 0.5 μl of a ...
Authors: Maria M. Gomzhina, Philipp B. Gannibal
Volume 2, Number 1, 2015
... influenza strains was performed by means of HAI and polymerase chain reaction (PCR). The hemagglutination and hemagglutination inhibition assays The hemagglutination and ...
Authors: Vera Krivitskaya, Evgeniy Sorokin, Tatyana Tsareva, Ekaterina Petrova, Nadezhda Konovalova, Maria Pisareva, Zhanna Buzitskaya, Tatyana Smirnova, Anna Sominina
Volume 3, Number 1, 2016
... gene and three overlapping parts of the HA gene were amplified by PCR using specific primers (sequence available upon request). The PCR products were purified using a Gel Band Purification Kit (Amersham ...
Authors: Andrea Wolkerstorfer, Dietmar Katinger, Julia Romanova
Том 4, номер 1, 2017
... этидия, извлекали из агарозного геля и очищали с помощью коммерческих наборов (ООО «Лаборатория Изоген» или Wizard PCR Preps DNA Purification Systems, A7170, Promega). Секвенирование ДНК ...
Authors: Р. Н. Гейдаров, Н. Ф. Ломакина, Е. Ю. Боравлева, И.С. Холодилов, А. С. Гамбарян, В. М. Михайлович, Е. Е. Фесенко
Volume 2, Number 1, 2015
... ) for a 9 month old child in Voronezh. The RNA of the virus A(H1N1)pdm09 was detected in the autopsy material by means of RT PCR (Fig. 4). All the other mortality cases were recorded during ...
Authors: Ludmila S. Karpova, Nina M. Popovtseva, Tatyana P. Stolyarova, Nadezhda I. Konovalova, Mikhail Y. Eropkin, Elena I. Burtzeva, Elena L. Feodoritova, Anna A. Sominina
Volume 4, Number 1, 2017
... fragments generated by reverse transcription followed by PCR were sequenced using 3130x/Genetic Analyzer (Applied Biosystems, USA) with BigDye Terminator V3.1 Sequencing Kit. GenBank accession numbers for the ...
Authors: Rustam N. Heydarov, Natalia F. Lomakina, Elizaveta Yu. Boravleva, Ivan S. Kholodilov, Alexandra S. Gambaryan, Vladimir M. Mikhailovich, Eugene E. Fesenko
Volume 5, Number 1, 2018
... the availability of the PCR technique, it became possible to identify the genes of the influenza virus in the autopsied and histological specimens of that time and to reconstruct the Spanish H1N1 ...
Authors: Andrej Egorov