ISSN 2500-2236
DOI-prefix: 10.18527/2500-2236

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Volume 3, Number 1, 2016
... chicken embryos by the genetic reassortment based on the cold-adapted A/Leningrad/134/17/57 (H2N2) master strain. The genome composition of the obtained reassortant was analyzed by means of real-time ...
Authors: Yulia A. Desheva, Tatiana A. Smolonogina, Galina O. Landgraf, Larisa G. Rudenko
Том 4, номер 1, 2017
... reassortants were analyzed by microarrays. The used microarrays contained 176 probes to the different segments of influenza virus genome. The microarray allows to determine 1) the hemagglutinin and ...
Authors: Р. Н. Гейдаров, Н. Ф. Ломакина, Е. Ю. Боравлева, И.С. Холодилов, А. С. Гамбарян, В. М. Михайлович, Е. Е. Фесенко
Volume 2, Number 1, 2015
... , Russia). The amplification of cDNA was performed by the standard method using the original primers. Sequencing of influenza virus A genome fragments (genes HA, NA, M, NS, and NP) was carried out on ABI ...
Authors: Mikhail P. Grudinin, Maria M. Pisareva, Andrey B. Komissarov, Anna A. Kosheleva, Zhanna V. Buzitskaya, Marina A. Stukova, Alexandra V. Prokopetz, Daria M. Danilenko, Nadezhda I. Konovalova, Tamara G. Lobova, Anna A. Sominina, Oleg I. Kiselev
Volume 4, Number 1, 2017
... ’-AGCAAAAGCAGG-3’). The fluorescently labeled PCR products were placed in the chamber. In the course of hybridization, the labeled fragments of the influenza virus genome formed a complex with the ...
Authors: Rustam N. Heydarov, Natalia F. Lomakina, Elizaveta Yu. Boravleva, Ivan S. Kholodilov, Alexandra S. Gambaryan, Vladimir M. Mikhailovich, Eugene E. Fesenko
Volume 3, Number 1, 2016
... the viral genome into the cytoplasm. Several adaptation passages are usually required to enable efficient influenza virus growth in Vero cells. A new cold adapted master strain for the cell line ...
Authors: Andrea Wolkerstorfer, Dietmar Katinger, Julia Romanova
Volume 4, Number 1, 2017
... , 8]. Prevention and proper treatment of influenza infection seem to be the best strategy to reduce secondary complications from influenza. In particular, it was found that influenza ...
Authors: Irina A. Leneva, Irina N. Falynskova, Nailya R. Makhmudova, Ekaterina A. Glubokova, Nadezhda P. Kartashova, Eugenia I. Leonova, Natalya A. Mikhailova, Irina V. Shestakova
Том 3, номер 1, 2016
... virus. The genome composition of the obtained reassortant was analyzed by means of real time PCR with the high resolution melting (HRM-analysis) using an intercalating fluorescent dye EvaGreen ...
Authors: Ю. А. Дешева, T. А. Смолоногина, Г. О. Ландграф, Л. Г. Руденко
Volume 3, Number 1, 2016
... The lack of population immunity to the periodically emerging pandemic influenza strains makes influenza infection especially dangerous. The fragmented nature of the influenza virus genome ...
Authors: Andrej Y. Egorov
Том 3, номер 1, 2016
... nature of the influenza virus genome contributes to the formation of influenza virus reassortants containing genomic fragments from different strains. This mechanism is one of the reasons for the ...
Authors: А.Ю. Егоров
Volume 2, Number 1, 2015
... was performed by the standard method using original primers. Sequencing of influenza virus A genome fragments (genes HA, NA, M, and NS) was carried out on ABI PRISM 3100-Avant Genetic Analyzer (Applied ...
Authors: Anna A. Sominina, Maria M. Pisareva, Mikhail P. Grudinin, Mikhail Yu. Eropkin, Nadezhda I. Konovalova, Vera F. Sukhovetskaya, Olga I. Afanasieva, Lubov V. Voloshuk, Andrey B. Komissarov, Zhanna V. Buzitskaya, Artem V. Fadeev, Kirill A. Stolyarov, Elizaveta A. Smorodintseva, Veronika S. Afanasieva, Marina A. Yanina, Vitalii V. Gonchar, Ludmila V. Osidak, Alexey A. Go
Volume 1, number 1, 2014
... best strategy to prevent virus spreading. Conventional inactivated vaccines (whole virus, split virus and subunit) made from these viruses were shown to have decreased content of immunocompetent ...
Authors: Mariia Sergeeva, Artem Krokhin, Mikhail Matrosovich, Tatyana Matrosovich, Markus Wolschek, Oleg Kiselev, Julia Romanova
Volume 5, Number 1, 2018
... removed NS1 protein reading frame, has successfully passed clinical trials [2]. The modification of the NS gene by the introduction of the foreign genome ...
Authors: Kirill A. Vasilyev, Maria A. Yukhneva, Anna-Polina S. Shurygina, Marina A. Stukova, Andrej Y. Egorov
Volume 5, Number 1, 2018
... additive effect on the reduction of virus shedding [21]. Tang et al. suggested that CRISPR/Cas9-based genome editing could be used as a powerful ...
Authors: Elizaveta Boravleva, Alexandra Gambaryan
Volume 3, Number 1, 2016
... against equine influenza, in addition to being safe and immunogenic, should also enable the differentiation of infected from vaccinated animals (DIVA strategy) [35 ...
Authors: Kaissar Tabynov
Volume 4, Number 1, 2017
... structural change happens in the endosome upon acidification and enables the fusion of the endosomal and viral membranes in order to release the viral genome to the cell cytoplasm. The pH threshold ...
Authors: Julia Romanova