ISSN 2500-2236
DOI-prefix: 10.18527/2500-2236

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Volume 5, Number 1, 2018
... Viruses Two strains based on the influenza A/Puerto Rico/8/1934 (H1N1) virus (A/PR8) encoding (1) the full-length NS1 protein (A/PR8/full NS) and (2) shortened to 124 ...
Authors: Kirill A. Vasilyev, Maria A. Yukhneva, Anna-Polina S. Shurygina, Marina A. Stukova, Andrej Y. Egorov
Том 4, номер 1, 2017
... neuraminidase proteins subtype; 2) the primary structure of the C-terminal sequence of the viral NS1 protein, which serves as a ligand for the PDZ domain; 3) the presence of stop codons and substitution N66S ...
Authors: Р. Н. Гейдаров, Н. Ф. Ломакина, Е. Ю. Боравлева, И.С. Холодилов, А. С. Гамбарян, В. М. Михайлович, Е. Е. Фесенко
Volume 4, Number 1, 2017
... A a Brief designation of the virus.b Amino acid at position 66 in the PB1-F2 protein.c The NS1 gene line ...
Authors: Rustam N. Heydarov, Natalia F. Lomakina, Elizaveta Yu. Boravleva, Ivan S. Kholodilov, Alexandra S. Gambaryan, Vladimir M. Mikhailovich, Eugene E. Fesenko
Volume 3, Number 1, 2016
... [6]. The polymerase complex PB2, PB1, and PA transcribes one mRNA from each genomic fragment, which is translated to the corresponding protein. In addition to M1 and NS1 proteins, two other ...
Authors: Andrej Y. Egorov
Volume 1, number 1, 2014
... preparations were diluted to a concentration of 15 µg/ml protein and treated with 100 µg/ml trypsin (TPCK-treated, Sigma) in PBS at pH 7.4 and 37°C for 2 h. Then, each sample was ...
Authors: Mariia Sergeeva, Artem Krokhin, Mikhail Matrosovich, Tatyana Matrosovich, Markus Wolschek, Oleg Kiselev, Julia Romanova
Volume 2, Number 1, 2015
... , 13, 14]. The role of interferon-induced transmembrane protein 3 (IFITM3) gene polymorphism in infectious pathology ...
Authors: O. I. Kiselev, A. B. Komissarov, O.S.Konshina, M.N.Dmitrieva, E.G.Deyeva, T.V.Sologub, V.I.Pokrovskiy
Том 5, номер 1, 2018
... The truncation of the nonstructural NS1 protein is a novel approach for the generation of immunogenic attenuated influenza viruses. However, the innate immune mechanisms that cause the increased ...
Authors: К. А. Васильев, М. А. Юхнева, А.-П. С. Шурыгина, М. А. Стукова, А. Ю. Егоров
Том 2, номер 1, 2015
... µg / ml the developed variant of cell-ELISA allows the detection of НА protein, synthesized in the cells infected with influenza A(H3N2) or A(H1N1)pdm09 virus, respectively.The developed method ...
Authors: В.З. Кривицкая, Е.В. Сорокин, Т.Р. Царева, Е.Р. Петрова, Н.И. Коновалова, М.М. Писарева, Ж.В. Бузицкая, Т.Д. Смирнова, А.А. Соминина
Volume 3, Number 1, 2016
... /Ham’s F12 medium (Biochrom F4815) with 4 mM L-glutamine and animal protein free supplement (proprietary formulation, Polymun Scientific GmbH, Austria). MDCK cells were cultivated in DMEM ...
Authors: Andrea Wolkerstorfer, Dietmar Katinger, Julia Romanova
Volume 2, Number 1, 2015
... concentration of 10-15 µg/ml, the developed variant of cell-ELISA was able to detect НА protein synthesized in the infected cells of influenza A(H3N2) and A(H1N1)pdm09 viruses, respectively ...
Authors: Vera Krivitskaya, Evgeniy Sorokin, Tatyana Tsareva, Ekaterina Petrova, Nadezhda Konovalova, Maria Pisareva, Zhanna Buzitskaya, Tatyana Smirnova, Anna Sominina
Volume 2, Number 1, 2015
... . No strains with resistance determining mutation S31N to rimantadine in the M2 protein were identified among clade 2B strains in contrast to rimantadine resistant strains of clade 2C ...
Authors: Mikhail P. Grudinin, Maria M. Pisareva, Andrey B. Komissarov, Anna A. Kosheleva, Zhanna V. Buzitskaya, Marina A. Stukova, Alexandra V. Prokopetz, Daria M. Danilenko, Nadezhda I. Konovalova, Tamara G. Lobova, Anna A. Sominina, Oleg I. Kiselev
Volume 4, Number 1, 2017
... envelope as well as organelles and DNA, mRNA, and tRNA molecules and their complexes that are formed in the course of the protein synthesis can be the targets for antibiotics (Fig. 2). The main ...
Authors: Alexander Zhivich
Volume 4, Number 1, 2017
... [9] and the US today is Optaflu/Flucelvax (Novartis) – the trivalent subunit vaccine against seasonal influenza. In addition, Protein Sciences ...
Authors: Julia Romanova
Том 1, номер 1, 2014
... мкг/мл; "Sigma") в течение 2 ч при 37°C. Затем пробы прогревали 5 мин при 95°C в буфере для проб и анализировали методом электрофореза в полиакриламидном геле (ПААГ). Содержание белка в пробах определяли методом Quant-iT Protein Assay ("Invitrogen"). ...
Authors: М.В. Сергеева, А. Крохин, М. Матросович, Т. Матросович, М. Волшек, О.И. Киселев, Ю.Р. Романова
Volume 5, Number 1, 2018
... strain of Salmonella typhimurium designed for the expression and delivery of H7N9 HA, NA, or the conserved extracellular domain of the matrix protein 2 (M2e) was constructed by Kim et al ...
Authors: Elizaveta Boravleva, Alexandra Gambaryan
Volume 5, Number 1, 2018
... thousands of interesting substances and samples. Hundreds of articles have been published, that, for example, led to discovery of selective inhibitors for G9a-like protein (GLP) lysine methyltransferase ...
Authors: Dmitri V. Debabov, Maria D. Debabova
Том 2, номер 1, 2015
... Chemoattractant Protein-1), IL-1β (Interleukin-1β), IL-6, IL-8, IL-10, ТNF-α (Tumor Necrosis Factor), IFN-γ и С-реактивный белок [27]. Авторы ...
Authors: О.И. Киселев, А.Б. Комиссаров, О.С. Коншина, М.Н. Дмитриева, Э.Г. Деева, Т.В. Сологуб, В.И. Покровский
Volume 3, Number 1, 2016
... immunization with the traditional inactivated vaccines, it is also possible to differentiate the infected from vaccinated animals through the detection of antibodies to the nonstructural NS1 protein of the ...
Authors: Kaissar Tabynov